Continuous acetone-butanol-ethanol (ABE) fermentation using immobilized cells of Clostridium acetobutylicum in a packed bed reactor and integration with product removal by pervaporation

  • Posted on: 12 June 2018
  • By: mmiltner
TitleContinuous acetone-butanol-ethanol (ABE) fermentation using immobilized cells of Clostridium acetobutylicum in a packed bed reactor and integration with product removal by pervaporation
Publication TypeJournal Article
Year of Publication1991
AuthorsFriedl A, Qureshi N, Maddox I
JournalBiotechnology and Bioengineering
Volume38
Issue5
Pagination518-527
KeywordsABE fermentation, Clostridiurn acetobutylicum, packed bed reactor, Pervaporation, Whey permeate
Abstract

An integrated solvent (ABE) fermentation and product removal process was investigated. A stable solvent productivity of 3.5 g/L h was achieved by using cells of Clostridium acetobutylicum immobilized onto a packed bed of bonechar, coupled with continuous product removal by pervaporation. Using a concentrated feed solution containing lactose at 130g/L, a lactose value of 97.9% was observed. The integrated fermentation and product removal system, with recycling of the treated fermentor effluent containing only low amount of solvents (/but lactose and acids), leads to only low acid losses. Therefore, most of the acids are converted to solvents, and this results in a high solvent yield of 0.39 g solvents/g lactose utilized. The pervaporation system provided a high product removal rate even at low solvent concentrations. A solvent membrane flux of 7.1 g/m(2) h with a selectivity of 5 was achieved during these investigations. The system proved to be very reliable.

URLhttps://www.ncbi.nlm.nih.gov/pubmed/18604810
DOI10.1002/bit.260380510